In a nutshell.....no!
By the time you're printing, the object or objects have been processed by the slicer and the gcode stream of instructions have been generated and written to file. The printing part of the cycle is simply sending these already written gcode instruction from that file to the printer one by one..
And if you're thinking that printing via USB is different to printing from a SD card....then they're not. Via USB, the gcode instructions are read by the software and squirted one by one to the printer. From a SD card, the printer does exactly the same thing.