what kind of printer are you using? If it's a RepRap style printer where you flash your own firmware, make sure the steps per mm is correct. You can use the Prusa Calculator to figure out what your steps per mm should be based off your hardware specifications:
http://prusaprinters.org/calculator/
If it's a non RepRap style printer just go onto tweaking the settings below
Once you have the proper steps per mm, you then want to tune extrusion multiplier, extrusion width, and filament diameter to get the calibration close to perfect.