I have a Prusa Mendel type with a Duet 0.8.5 controller, using Pronterface I can run each axis without problem.
The printer is connected to a Lenovo T510 running 64 bit Win7 Ult.
Initially I could't get Simplify3D to connect, but a couple of posts on this forum led me to uncheck the option 'Wait for startup command' in the menu Tools - Firmware Configuration - Communications. Interestingly this option is not saved between sessions. With this option unchecked communications with the printer were established.
However trying to test the comms by using the jog controls (Tools - Machine Control Panel - Jog Controls) results in one movement only, sometimes not even that. I can see the Gcode being generated but the printer does not respond. As I said at the top of this post using Pronterface the machine responds as expected.
I chose to try Simplfy3d having seen the demo video and was impressed by the fine tuning available and setup choices. However it's all to not avail if I can't connect reliably.
All help gratefully received,
Thanks,
Chris